Members - The Glycoscience Network

							Universidad del País Vasco
							Bilbao
Spain
						
email Emilio J. Cocinero# tools development
# academic research
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										Universidad del País Vasco
									

										
									
Our research interestsStructural properties of molecules and clusters are interrogated by
probing their electronic, vibrational and rotational energy levels using
various spectroscopic methods: pulsed laser spectroscopy (nanosecond),
high resolution rotational spectroscopy. Biological systems relevant to
biomolecular building blocks are our targets, such as carbohydrates,
peptides, glycopeptides as well as drugs for which structure-activity
relationship and molecular recognition processes are key factors that
govern their activity.
Selection of recent publications- Ribose Found in the Gas Phase										

										Angew. Chem. Int. Ed., 51, pp.
3119-3124 (2012)
										
- Probing the C-H···π Weak Hydrogen Bond in Anesthetic Binding: The Gasphase
Molecular Structure of the Sevoflurane-Benzene Cluster										

										Angew.
Chem. Int. Ed., 53, pp.3210-3213 (2014)
										
- Sensing the anomeric effect in a solvent-free environment										

										Nature, 469, pp.
76-80 (2011)
										
Our expertise- FTMW and CP-FTMW (Fourier transform Microwave spectroscopy)
- Electronic Laser Spectroscopy

																				language http://grupodeespectroscopia.es/MW/
																			

							Unité de Glycobiologie Structurale et Fonctionnelle
							Université de Lille - CNRS
France
						
email Fabrice Allain# academic research
# tools needed
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										Unité de Glycobiologie Structurale et Fonctionnelle
									

										
									
Our research interestsAt the UGSF, our team “Diversity of heparan sulfate (HS) and
inflammatory responses” is working on the regulation of expression and
activity of HS sulfotransferases, with the objective to elucidate the
relations structure/function of these glycosaminoglycans in inflammatory
and immune responses. We focus our interest on HS 3-Osulfafotransferases
(HS3STs) and explore the mechanisms by which
these enzymes generate functional HS structures in response to changes
in cellular environment. We have also developed in vitro enzymatic assay
for 3-O-sulfation, in order to produce HS mimetics that are closely
related to 3-O-sulfated HS. These molecule will allow us to analyse the
interaction between rare 3-O-sulfated motifs and their specific ligands.
Selection of recent publications- Macrophage polarization alters the expression and sulfation pattern of
glycosaminoglycans										

										Glycobiology, 25, pp.502-513 (2016)
										
- Molecular docking of heparin oligosaccharides with Hep-II heparinbinding
domain of fibronectin reveals an interplay between the different
positions of sulfate groups,										

										Glycoconj. J., 31, pp.161-169 (2014)
										
Our expertise- Identification of rare and functional sulfated sequences (density and
positions of sulfo groups) in heparan sulfates.

																			

							GlycoPedia
							
						
email Serge Perez# tools development
# academic research
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										GlycoPedia
									

										
									
Our research interestsThe Glycopedia experience focuses on glycoscience based topics to
popularize this scientific field and provides open access material to be
used for educational purposes. It provides a portal to share news
relevant to the community. They are multiform i.e. breaking news in the
fields, ‘managerial’ type of news, new books...
Selection of recent publications- The Symbolic Representation of Monosaccharides in the Age of
Glycobiology										

										
										
- NMR for Structural Glycoscience: I & II										

										
										
- Gas Chromatography - Mass Spectrometry for
Glycosciences										

										
										
- In situ Physico-Chemical Characterization of Surface
Confined Supramolecular Assemblies										

										
										
- An Overview of Human Innate Immunity										

										
										
- The Structure of Bacterial Cell Wall
NB: Many chapters are written in the context of PhD thesis.										

										
										
Our expertise- 3D Visualization of Complex Carbohydrates, Polysaccharides and
Glyco-Conjugates
- A Picture Dictionnary of Monosaccharide Symbols
- Library of Monosaccharide Templates. 1D, 2D, 3D
Structures
- Library of Bio-active Monosaccharides. 1D, 2D, 3D
Structures
- Online Databases & Tools

																			

							Institut Lumière Matière
							Université Lyon 1 - CNRS
France
						
email Isabelle Compagnon# tools development
# academic research
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										Institut Lumière Matière
									

										
									
Our research interestsAt Institut Lumière Matière, we are specialized in the development of
methods and instruments based on molecular physics for the structural
characterization of biomolecules in the gas-phase. With the coupling of
mass spectrometry with optical spectroscopy (IR, UV), we are able to
retrieve the structure of unmodified oligosaccharides from natural or
synthetic sources with atomistic resolution, using low amount of sample
and minimal purification.
Selection of recent publications- Anharmonic simulations of the vibrational spectrum of sulfated compounds:
application to the glycosaminoglycan fragment glucosamine 6-sulfate,										

										Phys.
Chem. Chem. Phys., 17, pp.25705-25713 (2015)
										
- Distinguishing isobaric phosphated and sulfated carbohydrates by coupling of
mass spectrometry with gas phase vibrational spectroscopy,										

										Phys. Chem.
Chem. Phys, 2014, 16, pp.22131-22138
										
Our expertise- Analysis of monosaccharide content, stereochemistry, regiochemistry
- Analysis of functional modifications and sulfate patterns in
glycosaminoglycans

																				language http://ilm.univ-lyon1.fr/index.php? option=com_content&view=article&id=221&act=207
																			

							Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
							Université Lyon 1 - INSA - CNRS
France
						
email Stephane Chambert# academic research
# tools needed
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										Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
									

										
									
Our research interestsIn team COB of ICBMS, the Organic and Bioorganic Chemistry
Laboratory at INSA-Lyon, we are interested in the design and
synthesis of carbohydrate containing molecules. Between other
projects, our research encompass the production of fluorescent
glycoprobes for biological imaging as well as pure standards for infrared
multiphoton analysis.
Selection of recent publications- Anharmonic simulations of the vibrational spectrum of sulfated compounds:
application to the glycosaminoglycan fragment glucosamine 6-sulfate,										

										Phys. Chem. Chem. Phys., 17, pp.25705-25713 (2015)
										
- Red Emitting Neutral Fluorescent Glycoconjugates for Membrane Optical
Imaging.,										

										Bioconjugate Chem., 25, pp.773-787 (2014)
										
Our expertise- Development of a fast analysis method for complex carbohydrate
containing samples

																			

							Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
							Université Lyon 1 - INSA - CNRS
France
						
email Sébastien Vidal# academic research
# tools needed
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										Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
									

										
									
Our research interestsIn Team CO2GLYCO, we design and synthesize glycoclusters as
multivalent carbohydrate-coated molecules. The core structures used
are calixarenes, pillararenes, fullerenes, porphyrins or resorcinarene. The
synthetic strategy is based on azide-alkyne “click” chemistry and simple
building blocks. The glycoclusters are then analyzed by NMR and mass
spectrometry for their characterization and homogeneity.
These glycoclusters are high affinity ligands of bacterial lectins and
applications in antibacterial therapy in vivo has been demonstrated.
Selection of recent publications- Pillar[5]arene-Based Glycoclusters: Synthesis and Multivalent Binding to
Pathogenic Bacterial Lectins,										

										Chem. Eur. J.,22, pp.2955–2963 (2016)
										
- Glycomimetics versus Multivalent Glycoconjugates for the Design of
High Affinity Lectin Ligands										

										Chem. Rev., 115, 525–561 (2015)
										
- Anti-adhesive properties of glycoclusters against Pseudomonas
aeruginosa lung infection										

										J. Med. Chem., 57, 10275–10289 (2014)
										
Our expertise- Some glycoclusters based on porphyrins are more difficult ot analyze
by ESI-MS since the molecular ion was decomposed. MALDI-MS was the
only technique possible and with strict conditions. A more universal
technique and easily applicable would be useful.

																			

							Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
							Université Lyon 1 - INSA - CNRS
France
						
email Sylvie Ricard-Blum# academic research
# tools needed
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										Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
									

										
									
Our research interestsThe team "Pericellular and Extracellular Supramolecular Assembly" builds and characterizes extracellular matrix interaction networks regulating angiogenesis, Alzheimer’s disease and host-pathogen interactions using in vitro and in silico approaches to decipher the molecular mechanisms involved. We focus on the structure, interactions and functions of multimolecular complexes anchored via glycosaminoglycans (GAGs) and syndecans in the pericellular matrix .
We need new analytical tools for the analysis of sulfate patterns and iduronic acid level in cell and tissue glycosaminoglycans, analysis of glycosaminoglycan sequences, and standard bioinformatic tools for glycosaminoglycans
Selection of recent publications- Launay et al. MatrixDB, the extracellular matrix interaction database:
updated content, a new navigator and expanded functionalities.										

										Nucleic
Acids Res, 43:D321-7 (2015)
										
- Peysselon & Ricard-Blum, Heparin-protein interactions: from affinity and
kinetics to biological roles. Application to an interaction network regulating
angiogenesis										

										Matrix Biol, 35:73-81 (2014)
										
- Fatoux-Ardore et al. Large-scale investigation of Leishmania interaction
networks with host extracellular matrix by surface plasmon resonance
imaging.										

										Infect Immun, 82:594-606 (2014)
										
- matrixdb.univ-lyon1.fr										

										MatrixDB, a protein-GAG interaction database 
										
Our expertise- Characterization of protein-protein and protein-GAG interactions by SPR and BioLayer Interferometry (kinetics, affinity)
tissue
glycosaminoglycans
- Curation of protein-protein and protein-GAG interactions
- MatrixDB (matrixdb.univ-lyon1.fr), a protein-GAG interaction database 
- Topological and functional analysis of protein-protein and protein-GAG interaction networks

																				language http://matrixdb.ibcp.fr
																			

							Centre de Recherche sur les Macromolécules Végétales
							CNRS Grenoble
France
						
email Sébastien Fort# tools development
# academic research
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										Centre de Recherche sur les Macromolécules Végétales
									

										
									
Our research interestsThe team Chemistry and Biotechnology of Oligosaccharides at CERMAV is
specialized in the synthesis of oligo(poly)saccharides and
glycoconjugates by chemical, enzymatic and microbial combined
approaches
Skills developed: CAZyme (Carbohydrate Active Enzymes) engineering,
Metabolic engineering of microorganisms, Biocatalysis and Green
Chemistry, Structural analysis
Selection of recent publications- Efficient chemoenzymatic synthesis of lipo-chitin oligosaccharides as
plant growth promoters,										

										Green Chem., 17(7), pp.3923-3930 (2015)
										
- Structure of an amino acid-decorated exopolysaccharide secreted by a Vibrio alginolyticus strain,										

										Mar. Drugs, 13(11), pp.6723-6739 (2015)
										
- Production of intracellular heparosan and derived oligosaccharides by
lyase expression in metabolically engineered E. coli K-12										

										Carbohydr.
Res., 360, pp.19-24 (2012)
										
Our expertise- Analysis of neutral and charged oligo- and polysaccharides

																				language http://www.cermav.cnrs.fr/en/chemistry-and-biotechnology-ofoligosaccharides
																			

							Swiss Institute of Bioinformatics
							Geneva
Switzerland
						
email Frédérique Lisacek# tools development
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										Swiss Institute of Bioinformatics
									

										
									
Our research interestsThe Proteome Informatics Group is involved in software and database development for the proteomics and the glycomics communities. The group focuses on mass spectrometry data analysis, the discovery of posttranslational modifications, and the functional study of glycoconjugates and carbohydrates. 
Selection of recent publications- MzJava: an open source mass spectrometry library										

										Horlacher O, Nikitin F, Alocci D, Mariethoz J, Müller M, Lisacek F (2015), J. Proteomics 129:63-70

										
- Graph Database vs RDF Triple Store: A Comparison on Glycan Substructure Search										

										Alocci D, Mariethoz J, Horlacher O, Bolleman JT, Campbell MP, Lisacek F (2015) PLoS One 10(12): e0144578 

										
- SugarBindDB, a resource of glycan-mediated host-pathogen interactions										

										Mariethoz J, Khatib K, Alocci D, Campbell MP, Karlsson NG, Packer NH, Mullen EH, Lisacek F (2016) Nucleic Acids Res. 44(D1):D1243-50

										
Our expertise- bioinformatics resource development
- mass spectrometry data analysis
- http://www.expasy.org/glycomics 

																			

							Manchester Institute of Biotechnology
							University of Manchester
UK
						
email Sabine L. Flitsch# tools development
# academic research
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										Manchester Institute of Biotechnology
									

										
									
Our research interestsCarbohydrates (such as sugars, glycans and polysachharides) provide the largest biomass on Earth and are central to many aspects of biotechnology. As such glycoscience and glycobiotechnology have broad applications in the development of biofuels, biomaterials and food stuffs from recycled and readily available biomass, as well as in medical research. Through investigation of the chemistry, structure and interactions associated with various carbohydrate molecules, it is possible to understand mechanisms of disease, develop novel glyco-based therapeutics and replicate the natural biosynthesis of glycans for synthetic purposes. However, the complex and diverse molecular nature of carbohydrates means that there is an urgent need to develop robust, wide-ranging synthetic and analytical methodologies for general use in this area. 

In the Flitsch lab. we have developed, and are continuing to expand, a toolbox for the synthesis and analysis of complex carbohydrates in order to understand their function. 
Selection of recent publications- Research article										

										P. Both, H. Busch, P. P. Kelly, F. G. Mutti, N. J. Turner, S. L. Flitsch, Sabine J. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION., 55; 1511 - 1513. DOI: 10.1002/anie.201510028 - JAN 22 2016
										
- Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing										

										Both, P.;   Green, A. P.;   Gray, C. J.;   Sardzik, R.;   Voglmeir, J.;   Fontana, C.;   Austeri, M.;   Rejzek, M.;   Richardson, D.;   Field, R. A.;   Widmalm, G.;   Flitsch, S. L.;   Eyers, C. E.  NATURE CHEMISTRY. 6(1); 65 - 74. DOI 10.1038/NCHEM.1817 - JAN 2014
										
- Accelerated Enzymatic Galactosylation of N-Acetylglucosaminolipids in Lipid Microdomains										

										Noble, Gavin T.;   Craven, Faye L.;   Voglmeir, Josef;   Sardzik, Robert;   Flitsch, Sabine L.;   Webb, Simon J. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 134(31); 13010 - 13017. DOI 10.1021/ja302506t - AUG 8 2012
										
- Chemoenzymatic Synthesis of O-Mannosylpeptides in Solution and on Solid Phase										

										- Sardzik, Robert;   Green, Anthony P.;   Laurent, Nicolas;   Both, Peter;   Fontana, Carolina;   Voglmeir, Josef;   Weissenborn, Martin J.;   Haddoub, Rose;   Grassi, Paola;   Haslam, Stuart M.;   Widmalm, Goran;   Flitsch, Sabine L. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 134(10); 4521 - 4524. DOI 10.1021/ja211861m - MAR 14 2012
										
- Enzymatic Amine Acyl Exchange in Peptides on Gold Surfaces										

										Castangia, Roberto;   Austeri, Martina;   Flitsch, Sabine L. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 51(52); 13016 - 13018. DOI 10.1002/anie.201205404 - 2012
										
- Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution										

										Rannes, Julie B.;   Ioannou, Avgousta;   Willies, Simon C.;   Grogan, Gideon;   Behrens, Carsten;   Flitsch, Sabine L.;   Turner, Nicholas J. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 133(22); 8436 - 8439. DOI 10.1021/ja2018477 - JUN 8 2011
										
Our expertise- (i)	glycoarrays to study glycoenzyme activity and discover carbohydrate-protein interactions 
- (ii)	ion mobility mass spectrometry for high resolution structural analysis of carbohydrates 
- (iii)	mass spectrometry for the label free identification of carbohydrate-binding proteins.
- (iv)	chemoenzymatic synthesis of glycoconjugates (glycopeptides, glycolipids, etc.)

																				language http://flitschlab.com
																			

							Institute of Integrative Biology
							University of Liverpool
UK
						
email Claire Eyers# tools development
# academic research
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										Institute of Integrative Biology
									

										
									
Our research interestsOur main interests lie in the development and application of biochemical and biophysical (mass spectrometry, ion mobility) techniques to study cellular signalling (proteins and their modifications, and sugars) both qualitatively and quantitatively. Investigations into the behaviour and manipulation of these biomolecules (glycans, modified peptides, intact proteins) in the gas-phase and their analysis by ion mobility-mass spectrometry, allows us to better derive useful information on their structure and interactions, which is applied to systems of particular interest, two key examples being biologics (biological therapeutics) and the NF-κB signalling pathway. 
Selection of recent publications- The power of ion mobility-mass spectrometry for structural characterization and the study of conformational dynamics										

										Lanucara F, Holman SW, Gray CJ, Eyers CE. Nat Chem. 2014 Apr;6(4):281-94. doi: 10.1038/nchem.1889.
										
- Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing										

										Both P, Green AP, Gray CJ, Sardzík R, Voglmeir J, Fontana C, Austeri M, Rejzek M, Richardson D, Field RA, Widmalm G, Flitsch SL, Eyers CE. Nat Chem. 2014 Jan;6(1):65-74. doi: 10.1038/nchem.1817
										
Our expertise- qualitative and quantitative analysis of protein post-translational modifications
- glycan structural analysis by IM-MS

																				language http://www.liverpool.ac.uk/cpr
																			

							LAMBE
							Université Evry Val d’Essonne - CNRS
France

						
email Régis Daniel# tools development
# academic research
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										LAMBE
									

										
									
Our research interests* Structural and functional analysis of biomolecules (proteins, carbohydrates), more particularly focused on:
-	Proteins of the human complement system
-	Carbohydrate-using enzymes (Discovering and studies of new carbohydrate specific enzymes and biotechnological applications)
-	Structure/activity relationship of bioactive polysaccharide glycosaminoglycans (GAGs) and sulfated polysaccharides from marine algae (Roles of GAGs in the regulation of the innate immunity, Complement system).
-	Structural characterization of isomeric sugars through metal complexation.

* Development of mass spectrometry hyphenated methods for deciphering information encoded in GAGs sequence, questioning the specificity of their molecular interactions, and probing structural determinants involved in their specific interaction with target proteins.
-	Affinity Capillary electrophoresis on-line coupled with ESI-MS
-	Surface Plasmon resonance coupled with MALDI-MS
-	Synchrotron X-Ray biomolecule footprinting coupled with ESI-MS
-	Liquid chromatography - Ion Mobility Mass spectrometry
Selection of recent publications- MALDI-TOF MS and ESI-LTQ-Orbitrap tandem mass spectrometry reveal specific porphyranase activity from Pseudoalteromonas atlantica bacterial extract										

										RSC Advances 5: 80793-80803 (2015)
										
- Effects of Calcium complexation on Heparin-like disaccharide. A combined theoretical, MS/MS and UV experiments										

										, Rapid. Commun. Mass Spectrom. 29:1135-1144 (2015)
										
- Biomarkers probed in saliva by surface plasmon resonance imaging coupled to maldi mass spectrometry in array format										

										Anal. Bioanal. Chem. 407:1285–1294 (2015)
										
- Single Molecule Detection of Glycosaminoglycan Hyaluronic Acid Oligosaccharides and Depolymerization Enzyme Activity Using a Protein Nanopore										

										ACS Nano 6: 9672-9678 (2012). 
										
- Probing the solution structure of Factor H using hydroxyl radical protein footprinting and cross-linking										

										Biochem. J., 2016, 473, 1805-1819.
										
- The chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from marine bacterium Vibrio sp FC509 is a dimeric species: biophysical characterization of an endosulfatase										

										Biochimie, 2016, 131, 85-95.
										
- Efficient recovery of glycosaminoglycan oligosaccharides from polyacrylamide gel electrophoresis combined with mass spectrometry analysis										

										Anal. Bioanal. Chem., 2017 409(5),1257-1269
										
- SOLEIL shining on the solution-state structure of biomacromolecules by synchrotron X-ray footprinting at the Metrology beamline										

										J. Synchr. Rad., 2017 24, 576-585
										
Our expertise- Capillary electrophoresis
- Surface Plasmon resonance on array biochips
- ion mobility-MS coupling
- Dynamic protein footprinting by X-ray synchrotron-mediated hydroxyl radical footprinting combined with high-resolution mass spectrometry 
- Proteomics and Glycomics
- Biomolecular Interactions
- Mass spectrometry

																				language http://www.lambe.univ-evry.fr/spip.php?article83
																			

							LAMBE
							Université Evry Val d’Essonne - CNRS
France

						
email Marie-Pierre Gaigeot# tools development
# academic research
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										LAMBE
									

										
									
Our research interestsDFT-MD (AIMD, FPMD) for theoretical anharmonic vibrational spectroscopy, CID, theoretical MS/MS
Structure characterization from FPMD & theoretical spectrosocopy

										Selection of recent publications
																			
Our expertise- DFT-based MD (DFT-MD)
- First-Principles MD (MD
- Ab initio MD (AIMD)

																				language http://www.lambe.univ-evry.fr/spip.php?article84
																			

							Bio-shape
							Bio-shape Ltd.
UK
						
email Hannah Roberts# private sector
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										Bio-shape
									

										
									
Our research interestsBio-Shape has developed cutting edge technology based on mass spectrometry techniques that enables the analysis of biomolecules and their behaviours under stresses, in exquisite unprecedented detail and very quickly.
OUR RESEARCH INTERESTS:
Studying the structure-function relationships between carbohydrates and proteins using glycoarrays
Sequencing carbohydrate structures 
Analysis of oligosaccharides extracted from fermentation waste for value added product streams
Investigating the activity and selectivity of glycoenzymes
Developing biocatalytic cascades, particularly for glycoconjugate synthesis and formal C-H amination
Supporting the discovery and production of small molecule and protein-based therapeutics
Investigation of ligand binding, conformational change, protein-protein interactions, aggregation, protein dynamics, binding stoichiometry, epitope mapping.
Expertise in the analysis of monoclonal antibodies (mAbs) and their derivatives.
Selection of recent publications- Dynamics of intact immunoglobulin G explored by drift-tube ion-mobility mass spectrometry and molecular modeling										

										DOI: 10.1002/anie.201402863
										
- Molecular Insights into the Thermal Stability of mAbs with Variable-Temperature Ion-Mobility Mass Spectrometry										

										DOI: 10.1002/cbic.201500574
										
- Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing										

										DOI: 10.1002/cbic.201500574
										
- Comprehensive stereochemical sequencing of carbohydrates using hyphenated mass spectrometry methods										

										BBA 2016 (Article in Press)
										
Our expertise- Mass Spectrometry 
- Ion Mobility Spectrometry 
- Glycoarrays 
- Glycoenzymes

																				language http://www.bio-shape.com
																			

							Elicityl
							Grenoble
France
						
email Benoît Darblade# tools needed
# private sector
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										Elicityl
									

										
									
Our research interestsBased near Grenoble, France, ELICITYL is a biotech company devoted to complex sugar engineering and production.The company mission is to supply oligosaccharides and polysaccharides for biological applications targeting plant health, pharmacy, nutraceutics and cosmetics.
Selection of recent publications- OligoTech®: a Library of complex carbohydrates & glycoproductsExtracted from biomassProduced by bacterial fermentationPotentially modified thru chemical functionalizationThe OligoTech® offer gives business opportunities using the untapped variety of complex sugars in compliance with all regulation appliances.										

										
										
- Complex sugars for Plant health: ELICITYL commercialises PEL101GV®, a complex sugar extracted from biomass enhancing the natural vine resistance to spring frosts.										

										
										
Our expertise- Analytical tools

																				language http://www.elicityl-oligotech.com
																			

							FELIX Laboratory
							Institute for Molecules and Materials - Radboud University 
Netherlands
 

						
email Jos Oomens# academic research
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										FELIX Laboratory
									

										
									
Our research interestsStructural elucidation by IR ion spectroscopy of mass-selected species in MS, including their MSn reaction products. Our main focus is on molecules of biological interest, peptide fragmentation products, polyaromatic species of astrophysical interest and applications in bio-analytical chemistry

										Selection of recent publications
																			
Our expertise- The FELIX free electron laser provides tunable, high intensity IR light at wavelengths between 3 and 150 mm. Tandem mass spectrometers available include FTMS and ion trap MS with ESI ion sources.

																				language http://www.ru.nl/msd
																			

							KTH Biotechnology/Glycoscience Division
							KTH Royal Institute of Technology
 
Stockholm, Sweden

						
email Francisco Vilaplana# academic research
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										KTH Biotechnology/Glycoscience Division
									

										
									
Our research interestsComplex carbohydrates (‘glycans’ or ´polysaccharides’) are fundamental biomolecules responsible for many regulatory processes in life sciences. They constitute the main energy-reserve molecules and they provide structural stability in eukaryotic cell walls. Polysaccharides constitute moreover the main components of biomass and therefore the main renewable resource for human and animal nutrition, biofuels and material production. The structural heterogeneity of native polysaccharides at the different hierarchical levels is fundamental for their specific functionality in biological tissues; however, this structural complexity imposes major difficulties for their biotechnological exploitation.

The complete structural determination of native polysaccharides at the molecular level is far from being implemented, as it is routinely done for the primary structure of nucleic acids and proteins. We are developing analytical tools for deciphering the molecular structure of complex biomass glycans (polysaccharides) using selective carbohydrate-active enzymes, multidimensional chromatography and mass spectrometry. This structural understanding can be directly related to their biosynthesis, remodeling and self-assembly in cell walls, their biological function, and their macroscopic properties (e.g. digestibility, solubility, aggregation, rheological and mechanical properties). This has fundamental implications for biomass deconstruction for biofuel production, for the design of functional carbohydrate-based materials, and for understanding the nutritional value of carbohydrate-rich foods.
Our research interest include:
(i)	MS-based glycomic methods for carbohydrate structural elucidation
(ii)	Understand the molecular structure and supramolecular architecture of eukaryotic cell walls (plants and fungi)
(iii)	Application of carbohydrate-active enzymes in biomass processing ('biorefineries')
(iv)	Design of bioactive carbohydrate-based materials and additives.
(v)	Nutritional benefits of dietary polysaccharides
Selection of recent publications- Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes										

										Green Chemistry (2017), DOI: 10.1039/C6GC03473J 
										
- Profiling the substitution pattern of xyloglucan derivatives by integrated enzymatic hydrolysis, hydrophilic-interaction liquid chromatography and mass spectrometry										

										Journal of Chromatography A (2016), 1463, 110-120
										
- A GH115 α-glucuronidase from Schizophyllum commune contributes to the synergistic enzymatic deconstruction of softwood glucuronoarabinoxylan										

										Biotechnology for Biofuels (2016) 9 2
										
- Two-Dimensional Size/Branch Length Distributions of a Branched Polymer										

										Macromolecules (2010) 43 7321
										

										Our expertise
																			

																				language http://www.biotech.kth.se/glycoscience/Francisco_Vilaplana
																			
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Universidad del País Vasco

BilbaoSpain

email Emilio J. Cocinero

Universidad del País Vasco

Our research interests

Selection of recent publications

Our expertise

Unité de Glycobiologie Structurale et Fonctionnelle

Université de Lille - CNRSFrance

email Fabrice Allain

Unité de Glycobiologie Structurale et Fonctionnelle

Our research interests

Selection of recent publications

Our expertise

GlycoPedia

email Serge Perez

GlycoPedia

Our research interests

Selection of recent publications

Our expertise

Institut Lumière Matière

Université Lyon 1 - CNRSFrance

email Isabelle Compagnon

Institut Lumière Matière

Our research interests

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Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

Université Lyon 1 - INSA - CNRSFrance

email Stephane Chambert

Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

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Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

Université Lyon 1 - INSA - CNRSFrance

email Sébastien Vidal

Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

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Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

Université Lyon 1 - INSA - CNRSFrance

email Sylvie Ricard-Blum

Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

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Centre de Recherche sur les Macromolécules Végétales

CNRS GrenobleFrance

email Sébastien Fort

Centre de Recherche sur les Macromolécules Végétales

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Swiss Institute of Bioinformatics

GenevaSwitzerland

email Frédérique Lisacek

Swiss Institute of Bioinformatics

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Manchester Institute of Biotechnology

University of ManchesterUK

email Sabine L. Flitsch

Manchester Institute of Biotechnology

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Institute of Integrative Biology

University of LiverpoolUK

email Claire Eyers

Institute of Integrative Biology

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LAMBE

Université Evry Val d’Essonne - CNRSFrance

email Régis Daniel

LAMBE

Bilbao
Spain

Emilio J. Cocinero

Université de Lille - CNRS
France

Fabrice Allain

Serge Perez

Université Lyon 1 - CNRS
France

Isabelle Compagnon

Université Lyon 1 - INSA - CNRS
France

Stephane Chambert

Université Lyon 1 - INSA - CNRS
France

Sébastien Vidal

Université Lyon 1 - INSA - CNRS
France

Sylvie Ricard-Blum

CNRS Grenoble
France

Sébastien Fort

Geneva
Switzerland

Frédérique Lisacek

University of Manchester
UK

Sabine L. Flitsch

University of Liverpool
UK

Claire Eyers

Université Evry Val d’Essonne - CNRS
France

Régis Daniel

Université Evry Val d’Essonne - CNRS
France

Marie-Pierre Gaigeot

Bio-shape Ltd.
UK

Hannah Roberts

Grenoble
France

Benoît Darblade

Institute for Molecules and Materials - Radboud University
Netherlands

Jos Oomens

KTH Royal Institute of Technology

Stockholm, Sweden

Francisco Vilaplana

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